Coding

Part:BBa_K3852005:Design

Designed by: Yuan Cao   Group: iGEM21_BIT-China   (2021-10-01)


T1R1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2491
    Illegal XbaI site found at 15
    Illegal XbaI site found at 854
    Illegal XbaI site found at 944
    Illegal XbaI site found at 1262
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2491
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2491
    Illegal BglII site found at 218
    Illegal BglII site found at 1820
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2491
    Illegal XbaI site found at 15
    Illegal XbaI site found at 854
    Illegal XbaI site found at 944
    Illegal XbaI site found at 1262
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2491
    Illegal XbaI site found at 15
    Illegal XbaI site found at 854
    Illegal XbaI site found at 944
    Illegal XbaI site found at 1262
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1440


Design Notes

In order to make the receptor better localize on the cell membrane of S. cerevisiae, the signal peptide sequence of Ste2 was added to its front end.


Source

Derived from the human genome

References